28: PFENNIG'S MEDIUM I

Modifications: plus 2% NaCl plus 0.2% MgSO4x7H2Oplus 0,3% Na-pyruvate

Growth observed:

Growth conditions: 3-7 days anaerobic in light 28 °C

1069: MODIFIED BIEBL AND PFENNIG'S MEDIUM

Modifications: For DSM 21808 and 21809. Adjust pH of final medium to 7.5. Add the following component after autoclaving from sterile stock solutions. Supplement medium with 1 ml/l Vitamin B12 (2 mg/l filter sterilised), 3 ml/l Na2S x 9 H2O (1M), 10 ml/l NaHCO3 (10%, autoclaved in sealed, half full vessels under CO2), and 3 g/l Sodium pyruvate.

Growth observed:

Growth conditions: 3-7 days anaerobic in light 28 °C